Related Information on MRC-5 Aborted Fetal Cell Line
| Cell Line Characteristics | |
| Repository Number: | AG06814 |
| Subcollection: | Specially Characterized Fibroblasts |
| Sample Description: | WI-38 – Normal Human Fetal Lung Fibroblast |
| DNA Sample: | NA06814 |
| Cell Type: | Fibroblast |
| Cell Subtype: | Lung |
| Genus species: | Homo sapiens |
| Common Name: | human |
| Clinically Affected: | No |
| Age: | 12 FW |
| Gender: | Female |
| Race: | Caucasian |
| Family Number: | 1053 |
| Relation to Proband: | proband |
| Remarks: | The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter. |
| ISCN: | 46,XX |
| Price: | $75 |
| Culture Protocol | |
| Split Ratio: | 1:5 |
| Temperature: | 37 C |
| Percent CO2: | 5% |
| Medium: | MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine |
| Serum: | 15% fetal bovine serum Uninactivated |
| Substrate: | None specified |
| Subcultivation Method: | trypsin-EDTA |
| Frozen at Passage: | 22 |
| Population Doubling at Freeze: | 22 |
| In vitro Lifespan: | Not determined |
| Split Ratio: | 1:4 |
| Temperature: | 37 C |
| Percent CO2: | 5% |
| Medium: | MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine |
| Serum: | 15% fetal bovine serum Uninactivated |
| Substrate: | None specified |
| Subcultivation Method: | trypsin-EDTA |
| Frozen at Passage: | 38 |
| Population Doubling at Freeze: | 44 |
| In vitro Lifespan: | Not determined |
| Split Ratio: | 1:5 |
| Temperature: | 37 C |
| Percent CO2: | 5% |
| Medium: | MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine |
| Serum: | 15% fetal bovine serum Uninactivated |
| Substrate: | None specified |
| Subcultivation Method: | trypsin-EDTA |
| Frozen at Passage: | 26 |
| Population Doubling at Freeze: | 34 |
| In vitro Lifespan: | Not determined |
| Characterizations | |
| IDENTIFICATION OF SPECIES OF ORIGIN: | Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
References
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| Kunisada T, Miller CD, Schneider EL, Mutat Res 237:75-81 (1990) Ultraviolet light-induced DNA damage in transcribed sequences: no change in repair with age. PubMed ID: 2164149 |
| Ganguly T, Duker NJ, Mutat Res 235:137-46 (1990) Differential cell cycle modulation of human DNA glycosylases against oxidized pyrimidines. PubMed ID: 2308590 |
| Pereira-Smith OM, Stein GH, Robetorye S, Meyer-Demarest S, J Cell Physiol 143:222-5 (1990) Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts. PubMed ID: 2332448 |
| Giordano T, Kleinsek D, Foster DN, Exp Gerontol 24:501-13 (1989) Increase in abundance of a transcript hybridizing to elongation factor I alpha during cellular senescence and quiescence. PubMed ID: 2483690 |
| McNearney T, Ballard L, Seya T, Atkinson JP, J Clin Invest 84:538-45 (1989) Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. PubMed ID: 2474570 |
| Giordano T, Foster DN, Exp Cell Res 185:399-406 (1989) Identification of a highly abundant cDNA isolated from senescent WI-38 cells. PubMed ID: 2480908 |
| Gibson JM, Milam SB, Klebe RJ, Mech Ageing Dev 48:101-10 (1989) Late passage cells display an increase in contractile behavior. PubMed ID: 2739467 |
| Kondo H, Kasuga H, Noumura T, Exp Cell Res 158:342-8 (1985) The heterogeneity of human fibroblasts as determined from the effects of hydrocortisone on cell growth and specific dexamethasone binding. PubMed ID: 4007059 |
| Hayflick, Exp Cell Res 37:614 (1965), The limited in vitro lifetime of human diploid cell strains. |
| Hayflick, Exp Cell Res 25:585 (1961), The serial cultivation of human diploid cell strains. |
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Dear GOD! Have mercy on this. Makes me want to vomit.