MRC-5 Aborted Fetal Cell Line

Coriell Cell Repositories
Related Information on WI-38 Aborted Fetal Cell Line

Cell Line Characteristics
Repository Number: AG05965
Subcollection: Specially Characterized Fibroblasts
Sample Description: MRC-5 – Normal Human Fetal Lung Fibroblast
Cell Type: Fibroblast
Cell Subtype: Lung
Genus species: Homo sapiens
Common Name: human
Clinically Affected: No
Age: 14 FW
Gender: Male
Race: Caucasian
Relation to Proband: proband
Remarks: The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B.
ISCN: 46,XY
Price: $75
Culture Protocol
Split Ratio: 1:5
Temperature: 37 C
Percent CO2: 5%
Medium: MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine
Serum: 15% fetal bovine serum Uninactivated
Substrate: None specified
Subcultivation Method: trypsin-EDTA
Frozen at Passage: Not determined
Population Doubling at Freeze: 27
In vitro Lifespan: Not determined
Split Ratio: 1:5
Temperature: 37 C
Percent CO2: 5%
Medium: MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine
Serum: 15% fetal bovine serum Uninactivated
Substrate: None specified
Subcultivation Method: trypsin-EDTA
Frozen at Passage: Not determined
Population Doubling at Freeze: 40
In vitro Lifespan: Not determined
Split Ratio: 1:12
Temperature: 37 C
Percent CO2: 5%
Medium: MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine
Serum: 15% fetal bovine serum Uninactivated
Substrate: None specified
Subcultivation Method: trypsin-EDTA
Frozen at Passage: Not determined
Population Doubling at Freeze: 17
In vitro Lifespan: Not determined
Characterizations
IDENTIFICATION OF SPECIES OF ORIGIN: Species of Origin Confirmed by Chromosome Analysis
IDENTIFICATION OF SPECIES OF ORIGIN: Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis

Publications

Guo WJ, Datta S, Band V, Dimri GP, Mel-18, a polycomb group protein, regulates cell proliferation and senescence via transcriptional repression of Bmi-1 and c-Myc oncoproteins Molecular biology of the cell18:536-46 2006
PubMed ID: 17151361
 
Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004
PubMed ID: 15459124
 
Skandalis A, Uribe E, A survey of splice variants of the human hypoxanthine phosphoribosyl transferase and DNA polymerase beta genes: products of alternative or aberrant splicing? Nucleic Acids Res32(22):6557-64 2004
PubMed ID: 15601998
 
Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001
PubMed ID: 11181576
 
Gyulai Z, Endresz V, Burian K, Pincus S, Toldy J, Cox WI, Meric C, Plotkin S, Gonczol E, Berencsi K, Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs. J Infect Dis181(5):1537-46 2000
PubMed ID: 10823751
 
Piret B, Schoonbroodt S, Piette J, The ATM protein is required for sustained activation of NF-kappaB following DNA damage. Oncogene18(13):2261-71 1999
PubMed ID: 10327072
 
Jongmans W, Vuillaume M, Chrzanowska K, Smeets D, Sperling K, Hall J, Nijmegen breakage syndrome cells fail to induce the p53-mediated DNA damage response following exposure to ionizing radiation. Mol Cell Biol17(9):5016-22 1997
PubMed ID: 9271379
 
Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995
PubMed ID: 7559606
 
Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995
PubMed ID: 7534307
 
Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line. Cancer Res54:1791-4 1994
PubMed ID: 7511052
 
Kilfeather SA, Collins D, McCormack P, Cotter T, O’Malley K, The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response. Mech Ageing Dev63:247-56 1992
PubMed ID: 1319530
 
Jacobs, Characteristics of a human diploid cell designated MRC-5. Nature277:168 (1970):247-56 1970
PubMed ID: 1319530
Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Cancer Res 54:1791-4 (1994) Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line.
PubMed ID: 7511052
Kilfeather SA, Collins D, McCormack P, Cotter T, O’Malley K, Mech Ageing Dev 63:247-56 (1992) The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response.
PubMed ID: 1319530
Jacobs, Nature 277:168 (1970), Characteristics of a human diploid cell designated MRC-5.